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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
to crystallize proteins has obviously improved, and this web page highlights a few of the methods currently available. Figure 2. Three-dimensional structure of porcine pepsin. Pepsin was the first globular protein crystal used successfully in x-ray
were passed through 1-ml nickel affinity columns (B-PER 6×His fusion protein purification kit from Pierce)that had been pre-equilibrated with 7 M GuHCl in TBS (0.15 M NaCl,50 mM Tris.HCl pH 7.5).The columns were washed thrice with 1 ml 7 M GuHCl
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