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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80℃储存
- 英文名:
Small RNA Ladder
- 库存:
询盘
- 供应商:
上海阿拉丁生化科技股份有限公司
- 规格:
S750554-100μl
英文名:Small RNA Ladder
纯度或规格:BioReagent,适用于电泳,分子生物学级,无菌,10-50nt, 9 bands
SPU:S750554
CAS:-
存储温度:-80℃储存;避免反复冻融
运输条件:特低温运输
查看阿拉丁官网此产品相关对应页面:
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文献和实验Cloning of small RNAs with 5’ phosphate and 3’ OH ends
of small RNA (from 10 to 35 µg total RNA). Note, we also use Arabidopsis floral tissue, which has a high percentage of small RNAs (comment 1). Prepare the 15% 8M UREA gel. Pour a 15% UREA gel in the Biorad MiniProtean 2, using 0.75mm spacers
system. The RNA bands may fade and disappear on prolonged exposure to UV light. 2. Glyoxal Gels 1) Denature 3 μg (3 μl) RNA Ladder in loading buffer (recommended final loading buffer concentration is 1 M deionized glyoxal, 50% (v/v) DMSO, 10
at lower concentrations. Because an A260 of 0.1 corresponds to ~4 µg/ml RNA, it is often impractical to use UV spectroscopy to quantitate RNA isolated from small samples that will have lower concentrations once diluted. Fortunately, there are alternative
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