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文献和实验【共享】如何检测GFP、GFP、YFP、EYFP、CFP抗体?
Antibody均能与GFP、YFP、EYFP、CFP等突变体有交叉反应,可以用于GFP、YFP、EYFP、CFP等荧光蛋白的表达定量、定位分析和检测。EarthOx该GFP标签抗体(货号#E022030)的另一个优势是,可用于的试验类型很多,除了基本的WB、ELISA试验外,还可以用于免疫细胞化学、免疫荧光(ICC/IF)、免疫共沉淀(IP)等。该抗体的效价很高,EarthOx的推荐WB起始稀释比率为1:1000,实际应用于中还能有更优异的表现。因此,EarthOx的Anti-GFP Tag
Chemotaxis in Neutrophil-Like HL-60 Cells
known as chemotaxis. In particular, leukocyte migration in the innate immune system has been studied in the human neutrophil-like cell line (HL-60). Here, we describe the maintenance and transfection of HL-60 cells and explain how to analyze
Isolation of Hair Follicle Bulge Stem Cells from YFP-Expressing Reporter Mice
In this article we provide a method to isolate hair follicle stem cells that have undergone targeted gene inactivation. The mice from which these cells are isolated are bred into a Rosa26- yellow fluorescent protein (YFP
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