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文献和实验consists of a cooperation between monocytes and cytophilic antibodies able to bind to the Fc receptors present on the monocyte surface. Thus human IgG1 and IgG3 are the main isotypes effective in ADCI, whereas IgG2, IgG4, and IgM are ineffective
Ultrasensitive ELISA for Measurement of Human Cytokine Responses in Primary Culture
ELISAs offer excellent specificity and, once fully optimized, sensitivity that rivals that of bioassays. The major variables that need to be experimentally determined when developing an ELISA are the optimal number of fresh cells required
thousands of colored products. Thus, in ELISA, antigens are detectable when present in only nanogram and picogram quantities. One form of the ELISA is carried out in a microtiter plate which is used as the solid phase for adsorption of antigen (Figure
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