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- 详细信息
- 文献和实验
- 技术资料
- 库存:
99
- 供应商:
南京赛泓瑞生物
- 检测方法:
ELISA
- 适应物种:
人
- 样本:
细胞培养上清、血清、血浆、尿液、组织样本等
- 规格:
48T/96T
产品名称:human phosphatidylinositol antibody IgG/IgM,PI Ab-IgG/IgM ELISA Kit
用途
该试剂盒用于体外定量检测人血清、血浆或其他相关生物液体中天然及部分human phosphatidylinositol antibody IgG/IgM,PI Ab-IgG/IgM ELISA Kit需自备的设备及试剂
1、450±10nm 滤光片的酶标仪(建议仪器使用前提前预热)
2、单道或多道微量加液器及吸头
3、稀释样品的EP 管
4、蒸馏水或去离子水
5、吸水纸
6、盛放洗液的容器
human phosphatidylinositol antibody IgG/IgM,PI Ab-IgG/IgM ELISA Kit
human phosphatidylinositol antibody IgG/IgM,PI Ab-IgG/IgM ELISA Kit标本的采集与保存:
human phosphatidylinositol antibody IgG/IgM,PI Ab-IgG/IgM ELISA Kit



















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文献和实验Protein A Purification of Antibody
fractions until base line is achieved.Elute Ab with 5 bed volumes of Elution Buffer. Collect 1 ml fractions into 50 ml 1M Tris, until base line is achieved.Take 50 ml aliquots of each fraction for ELISA.Pool fractions containing Ab. Dialyze against 2 x 4L
Protein A Purification of Antibody
with 5 bed volumes of Elution Buffer. Collect 1 ml fractions into 50 m l 1M Tris, until base line is achieved. Take 50 m l aliquots of each fraction for ELISA. Pool fractions containing Ab. Dialyze against 2 x 4L PBS
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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