sigma：Anti-Mouse IgG (Fab spec
ific)–Alkaline Phosphatase antibody produced in goat 货号：A2179-.5ML/A2179-1ML- ￥1860 - 3154
- sigma
- 美国
- A2179
- 2025年12月26日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
见瓶身
- 保质期：
见lot
- 库存：
999
- 供应商：
嘉兴亦高
- 规格：
A2179-.5ML/A2179-1ML
| 规格： | A2179-.5ML | 产品价格： | ￥1860.0 |
|---|---|---|---|
| 规格： | A2179-1ML | 产品价格： | ￥3154.0 |
Immunoglobulins (Igs) belongs to the immunoglobulin super-family. Each immunoglobin have two heavy (H) and two light (L) chains held together by disulfide linkages.
免疫原
Purified mouse IgG, Fab fragment
应用
Alkaline phosphatase-conjugated goat anti-mouse IgG (Fab specific) was used as the secondary antibody in ELISA assays to measure the reacitivity of mABs to thyrogobulin peptides. Absorbance was measured at 405 nm.
Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody produced in goat has been used in:
western blotting
immunoblotting
enzyme linked immunosorbent assay (ELISA)
immunohistochemistry
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文献和实验Ling XH, et al.
Oncology Letters, 11(3), 2001-2008 (2016)
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
phosphatase-conjugated antibodies. However, by carefully controlling the reaction time in X-gal so that only a small amount of indigo is produced (Vaysse and Goldman, 1990), by having ß-gal localized to the nucleus when the cellular antigen is non-nuclear
Expression of Recombinant Alkaline Phosphatase Conjugates in Escherichia coli
analysis, and competition or sandwich ELISAs (see Note 1 ). Expression of an scFv antibody fragment derived from an IgG2a/κ immunoglobulin specific for curaremimetic toxins from snake (named M-α2-3), will be used to illustrate the methods utilized
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
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