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- 详细信息
- 文献和实验
- 技术资料
- CAS号:
见产品外包装
- 英文名:
Anti-Human IgE (ε-chain specific)−Alkaline Phosphatase antibody produced in goat
- 供应商:
嵘崴达
- 库存:
需确认
- 保质期:
见产品外包装
- 保存条件:
2-8°C
- 规格:
0.5 mL/1 mL
| 规格: | 0.5 mL | 产品价格: | ¥2978.0 |
|---|---|---|---|
| 规格: | 1 mL | 产品价格: | ¥5793.0 |
Anti-Human IgE (ε-chain specific)−Alkaline Phosphatase antibody produced in goat
affinity isolated antibody, buffered aqueous glycerol solution
Application
Anti-Human IgE (epsilon-chain specific)-Alkaline Phosphatase antibody is suitable for use in immunoblotting and ELISA (at 1:500 dilutions using 100μ/well ).
Anti-Human IgE (ε-chain specific)−Alkaline Phosphatase antibody produced in goat has been used in dot blot.
IgE secreted from transfected murin myeloma cells was detected by ELISA using the alkaline phosphatase-conjugated goat anti-human IgE (epsilon specific) antibody.
Biochem/physiol Actions
IgE bound to B cell receptor mediates antigen uptake and regulates differentiation and proliferation of B cells.
Physical form
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 15 mM sodium azide and 50% glycerol
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
dehydrogenases. Progr. Histochem. Cytochem . 4 :225. Berger, J., A.D. Howard, L. Gerber, B.R. Cullen, and S. Udenfriend. 1987. Expression of active, membrane-bound human placental alkaline phosphatase by transfected simian cells. Proc. Natl. Acad. Sci
Secondary antibody: Goat anti-mouse alkaline phosphatase conjugated - Biorad #170-6520. Substrate: ECF chemifluorescent substrate - Amersham #RPN5785. Mix substrate with accompanying buffer as per manufacturer’s
and wash the blot 3 times, 5-7 minutes each, with 0.5% BSA in TBST.11. Incubate with Goat anti-Rabbit/alkaline phosphatase (from Promega; 1:7500 inTBST with 5% BSA) for 1 hour at room temp with shaking. I have also used successfully Amersham ECL detection
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