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文献和实验Receptor Tyrosine Kinase Inhibitor Profiling Using Bead-Based Multiplex Sandwich Immunoassays
) as an example. We provide a protocol for a bead-based sandwich immunoassay that enables a relative quantification of the EGFR and its generic tyrosine phosphorylation. We also present data from a kinase inhibitor experiment using 96-well cell-culture plates
Measurement of Phosphoinositide 3-Kinase Activity
class IA PI 3-kinase is a heterodimer consisting of the 85 kDa regulatory subunit (responsible for protein-protein interactions either via protein tyrosine phosphate-binding SH2 domains or SH3 domains and/or proline rich regions) and a catalytic 110 kDa
Targeting Cancer with Small-Molecular-Weight Kinase Inhibitors
kinase-targeted drugs are in clinical development and many more in various stages of preclinical development. Kinase inhibitor drugs that are in clinical trials target all stages of signal transduction from the receptor protein tyrosine kinases
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