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文献和实验【信息】The family of five: TIR-domain-containing adaptors in Toll-like receptor signalling
Review Nature Reviews Immunology 7, 353-364 (May 2007) | doi:10.1038/nri2079 The family of five: TIR-domain-containing adaptors in Toll-like receptor signalling Luke A. J. O'Neill1 & Andrew G. Bowie1 About the authors Summary TLR (Toll
us to change genomic sequences at will to control the fundamental geneticinformation within a cell. It has been implemented in creatingmodel cell lines or organisms for the study of gene functions orgenetics of human diseases, and in gene therapy
Gene Knockout In Murine Embryonic Stem (ES) Cells
and adding 4 ml of ES media containing 200µg/ml (active portion) G418. NB if you electroporated on Tuesday morning then you will have to come in that night and set up the selection. Day 2 - 10 (Thursday - Friday) Change the media (G418 ES
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