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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
in the developing striatum. Neuron 9 :15. Henthorn, P., P. Zervos, M. Raducha, H. Harris, and T. Kadesch. 1988. Expression of a human placental alkaline phosphatase gene in transfected cells: Use as a reporter for studies of gene expression. Proc. Natl
Da (116). (ii) Analysis of phosphopeptides by MALDI-TOF. MALDI-TOF mass spectrometry can also be used to identify phosphopeptides (81, 130, 177, 178). When phosphorylated peptides are subjected to ionization by MALDI, phosphate groups
Methods for the Detection of D-Amino-Acid Oxidase
al. (5 ) using bovine serum albumin as a standard. It was also determined using a Protein Assay Kit (Bio-Rad, Hercules, CA). D-Amino-acid oxidase activity is finally expressed as the amount of D-alanine oxidized per min per milligram of protein. Hog
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