PerCP Mouse IgG2b Isotype Cont

Rapid Isotyping of Mouse Monoclonal Antibodies

for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance

Purification of Lin-, c-kit+, Sca-1+ bone marrow cells for Culture, Flow Cytometry, or Transplantaion

D  Granulocytic : Gr-1 (Ly-6G) IgG2b, 01211A  D8 IgG2b, 09821D  Eryth: Ter-119 IgG2b, 09081A  Sca-1 PE-IgG2a (E31-161.7) 01835B  PE-IgG2a Isotype control, 11025

流式试剂选择及常见问题解答

样本 同型对照管：纯化的同型对照＋PE标记的抗Mouse IgG1＋样本 如果没有找到适合的同型对照，在保持来源相同、荧光标记相同、免疫球蛋白类别相同条件下，那么优先选择的顺序应该是亚链不同－－亚型不同－－相同类别。比如，某种的抗体的来源是Mouse IgG2а,κ。如果在同型对照表里面找到不到完全相同的同型对照，那么优先选择相同荧光标记的Mouse IgG2а为同型对照。如果也没有找到Mouse IgG2а，那么优先选择相同荧光标记的Mouse IgG2b