Blood group B antigen triose g

Purification of Sequence‐Specific DNA‐Binding Proteins by Affinity Chromatography

available CNBr?activated Sepharose, and a support protocol describes how to purify crude synthetic oligonucleotides by gel electrophoresis prior to preparation of the affinity resin. The second basic protocol outlines the affinity chromatography procedure

蛋白质组学指南

or are isolated by affinity chromatography. To accomplish this, we use affinity columns and other strategies to select for protein targets. In each case, protein samples are subjected to a series of precolumns and high-stringency washes to remove

Size and Shape of Protein Molecules at the Nanometer Level Determined by Sedimentation, Gel Filtration, and Electron Microscopy

of the purified protein complex and illustrates several important features. • For both the gel filtration (size exclusion chromatography, Fig.   5 a) and gradient sedimentation, Fig.   5 b, two calibration