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Anti-SV40 T-Antigen, clone PAb

416-L, Recombinant Mouse Monoclonal Ab
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  • ¥860
  • Elucigen Biosciences
  • EMS0119
  • 2025年08月13日
  • ICC, WB, Flow
  • Mouse
  • Virus
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  • 企业认证

    • 详细信息
    • 文献和实验
    • 技术资料
    • 亚型

      Mouse / IgG2a

    • 形态

      Liquid

    • 保存条件

      -20°C

    • 克隆性

      Monoclonal

    • 标记物

      Unconjugated

    • 适应物种

      Virus

    • 保质期

      12 months from the shipping date of the product.

    • 抗原来源

      Human

    • 目录编号

      EMS0119

    • 级别

      Primary Antibodies

    • 库存

      Available

    • 供应商

      Elucigen Biosciences

    • 宿主

      Mouse

    • 应用范围

      ICC, WB, Flow

    • 浓度

      0.5 mg/mL

    • 靶点

      P03070

    • 抗体名

      Anti-SV40 T-Antigen, clone PAb416-L, Recombinant Mouse Monoclonal Ab

    • 规格

      25μg/100μg/500μg

    规格:25μg产品价格:¥860.0
    规格:100μg产品价格:询价
    规格:500μg产品价格:询价
    SV40 T antigen (Simian Virus 40 Large T Antigen) is a viral protein encoded by the SV40 virus, a polyomavirus that infects primates. The T antigen plays a critical role in the virus's ability to replicate and transform host cells. It is a multifunctional protein that interacts with various cellular proteins, primarily through its binding to the tumor suppressor proteins p53 and RB (retinoblastoma protein), leading to their inactivation. By doing so, SV40 T antigen promotes cellular transformation, allowing the virus to bypass normal growth control mechanisms, facilitating both viral replication and potential tumorigenesis in infected cells. Because of its ability to induce cellular transformation and disrupt normal regulatory pathways, SV40 T antigen has been extensively used as a tool in molecular biology research, particularly in studies involving cell cycle regulation, DNA replication, and oncogenesis. Additionally, SV40 T antigen has contributed to the understanding of tumorigenic mechanisms and the role of key cell cycle regulators in cancer.

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    图标文献和实验
    该产品被引用文献
    Cheng, J. et al. (2009) Semin Cancer Biol 19, 218-28.
    相关实验
    • T-cell dependent antigen specific in situ staining (Pe)

      6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block

    • Recombinant DNA Engineering, Or Cloning Genes In Plasmids

      also usually have promoter segments for phage polymerases (like T7 and SP6 RNA polymerases) such that one can use in them vitro to produce RNA. A plasmid designed for mammalian expression also usually has a transcription termination and polyadenylation signal

    • Monoclonal Antibody Production

      (note the removal of the HT)in a T25 flask (one for each positive clone)for later use (7) When the cells are ready for expansion out of the 24-well plate,resuspend them and pipet to the T25 flasks with feeder cells.Again leave a little bit in the 24

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    文献支持
    Anti-SV40 T-Antigen, clone PAb416-L, Recombinant Mouse Monoclonal Ab
    ¥860