N-Biotinyl-3-aminopropyl Solke

Functional Analysis of Human D1 and D5 Dopaminergic G Protein-Coupled Receptors: Lessons from Mutagenesis of a Conserved Serine Residue in the Cytosol

In mammals, dopamine G protein-coupled receptors (GPCR) are segregated into two categories: D1-like (D1R and D5R) and D2-like (D2Rshort , D2Rlong , D3R, and D4R) subtypes. D1R and D5R are primarily coupled to stimulatory heterotrimeric GTP

Assay of N 8-Acetylspermidine Deacetylase

of the molecule. Thus, N 8 -acetylspermidine and N -acetylputrescine are substrates for this enzyme, whereas polyamines acetylated on aminopropyl groups, such as N 1 -acetylspermidine and N -acetylspermine, are not substrates (2 ). The metabolism

Cell Surface Engineering of Mesenchymal Stem Cells

a stepwise process including (1) treatment of cells with sulfonated biotinyl-N -hydroxy-succinimide to introduce biotin groups on the cell surface, (2) addition of streptavidin that binds to the biotin on the cell surface and presents unoccupied binding sites