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- 英文名：
1-Hydroxypyrene-d9 Beta-D-Glucuronide (100ug/mL in Methanol)
- 库存：
100+
- 供应商：
上海维亦特生物
- CAS号：
N/A
1-Hydroxypyrene-d9 Beta-D-Glucuronide (100ug/mL in Methanol)
N/A
C₂₂H₉D₉O₇
403.43
Colourless Solution
Methanol
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文献和实验1-Hydroxypyrene-d9 Beta-D-Glucuronide (100ug/mL in Methanol)
N/A
C₂₂H₉D₉O₇
403.43
Colourless Solution
Methanol
Sucrose Density Gradient Fractionation of Yeast Membranes
of growth) Wait 1 doubling period (about 2 hours) until O.D.600 equals about 1 Add 1 ml of COLD 1M NaN3 to 100 ml culture (we want 10 mM total conc.) Spin down 3 x 109 cells (about 100 O.D. equivalents (100 ml of OD = 1): 2750 X g
--one with 1 ml of the 2 ml culture, the other with 0.5 ml of the 2 ml culture Spin at 2750 X g. for 10 min. at 30oC Resuspend in 100 ml pre-warmed YPD so that O.D.600 = 0.5 (50 ml of O.D.=1 culture into 100 ml total YPD) If appropriate
Agar Plates for Selection of Clones in Bacteria
C (can be stored in dark at 4C but -20 is usually as simple!) X-Gal: 20 mg/ml in Dimethylformamide (DMF) IPTG: 100 mM Ampicillin: 100 ug/ml (micrograms) Filter sterilize X-Gal is 5-bromo-4-chloro-3-indolyl-beta-D-galactoside. It is hydolysed
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