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Benzyl 2-Acetamido-2-deoxy-4-O-Beta-D-galactofuranosyl-Alpha-D-glucopyranoside
N/A
C₂₁H₃₁NO₁₁
473.47
White Solid
Methanol, Water
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文献和实验Benzyl 2-Acetamido-2-deoxy-4-O-Beta-D-galactofuranosyl-Alpha-D-glucopyranoside
N/A
C₂₁H₃₁NO₁₁
473.47
White Solid
Methanol, Water
tumor necrosis factor–{alpha} (TNF-{alpha}), interferon-beta (IFN-beta), and IFN-{gamma} (1). The authors observed that of ~200 miRNAs examined, only miR-155 increased in amount in response to both TLR ligands and IFN-beta. miR-155 accumulated
Sucrose Density Gradient Fractionation
of O.D.=1 culture into 100 ml total YPD)If appropriate,after 1 hr add 2.5 uM alpha factor (421μl of stock (1 mg/ml)to 100 ml of culture)(we want it present during the last hour of growth)Wait 1 doubling period (about 2 hours)until O.D.600 equals
Sucrose Density Gradient Fractionation of Yeast Membranes
Resuspend in 100 ml pre-warmed YPD so that O.D.600 = 0.5 (50 ml of O.D.=1 culture into 100 ml total YPD) If appropriate, after 1 hr add 2.5 uM alpha factor (421 ul of stock (1 mg/ml) to 100 ml of culture) (we want it present during the last hour
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