- ￥3480 - 11180
- Sino Biological
- 北京
- 10780-H85H5-SD
- 2026年01月26日
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human CD70/CD27 Ligand Protein (Site-Specific AF 488 Conjugation, ECD, His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
25 µg/100 µg
| 规格： | 25 µg | 产品价格： | ￥3480.0 |
|---|---|---|---|
| 规格： | 100 µg | 产品价格： | ￥11180.0 |
蛋白名称：Human CD70/CD27 Ligand Protein (Site-Specific AF 488 Conjugation, ECD, His Tag)
蛋白构建：A DNA sequence encoding the human CD70 (NP_001243.1) (Gln 39-Pro 193) was expressed with a N-terminal polyhistidine tag. This construct has a trimer design.The protein is site-specifically conjugated with AF 488 (Excitation Max.= 495 nm, Emission Max.= 519 nm).
表达宿主：HEK293 Cells
蛋白纯度：≥ 90 % as determined by SDS-PAGE.
蛋白活性：1.Flow cytometric analysis of anti-CD70 CAR expression. 5e5 of anti-CD70 CAR-293 cells were stained with 100 μL of 6μg/ml of AF 488-conjugated human CD70 Protein (Site-Specific AF 488 Conjugation, ECD, His Tag, Cat. No. 10780-H85H5-SD) and negative control protein. Non-transduced 293 cells were used as a control (left). AF 488 signal was used to evaluate the binding activity (QC tested). 2.Binding activity of AF 488-conjugated CD70 protein to PBMC cells. PBMC cells were stained with PE conjugated anti-CD3 antibody and AF 488-conjugated human CD70 Protein (Site-Specific AF 488 Conjugation, ECD, His Tag, Cat. No. 10780-H85H5-SD) and detected by flow cytometry. PE signal was used to evaluate the content of CD3+ T cells in PBMCs. AF 488 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Gly
蛋白分子量：The protein has a predicted molecular mass of 54.8 kDa.
蛋白NP号：NP_001243.1
蛋白氨基酸序列：Gln39-Pro193
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Labeling Cell-Surface Proteins Via Antibody Quantum Dot Streptavidin Conjugates
such as streptavidin, quantum dots can be used to label different biotinylated antibodies or ligands without having to customize the quantum dot surface chemistry for each antibody or ligand. In this chapter, we outline the methodology for using streptavidin quantum
免疫组化技术-Immunohistochemical Protocols
it as a marker to search for the antigen in tissue. The antibody can be directly conjugated to a fluorochrome or biotin that can then be detected in several different ways. Much more common, however, is the use of a non-conjugated primary antibody
High‐Throughput, Multiplexed Analysis of 3‐Nitrotyrosine in Individual Proteins
. In addition, modifications in single?tissue?associated proteins released into blood can provide insight into the tissue localization of the inflammation or oxidative stress. We have developed an enzyme?linked immunosorbent assay antibody microarray platform
技术资料暂无技术资料 索取技术资料
