- ￥3480 - 11180
- Sino Biological
- 北京
- 10780-H85H5-SD
- 2025年08月12日
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- 详细信息
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human CD70/CD27 Ligand Protein (Site-Specific AF 488 Conjugation, ECD, His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
25 µg/100 µg
| 规格： | 25 µg | 产品价格： | ￥3480.0 |
|---|---|---|---|
| 规格： | 100 µg | 产品价格： | ￥11180.0 |
重组人CD70/CD27 Ligand蛋白(AF 488-conjugated)（产品说明）
蛋白名称：Human CD70/CD27 Ligand Protein (Site-Specific AF 488 Conjugation, ECD, His Tag)
蛋白构建：A DNA sequence encoding the human CD70 (NP_001243.1) (Gln 39-Pro 193) was expressed with a N-terminal polyhistidine tag. This construct has a trimer design.The protein is site-specifically conjugated with AF 488 (Excitation Max.= 495 nm, Emission Max.= 519 nm).
表达宿主：HEK293 Cells
蛋白纯度：≥ 90 % as determined by SDS-PAGE.
蛋白活性：1.Flow cytometric analysis of anti-CD70 CAR expression. 5e5 of anti-CD70 CAR-293 cells were stained with 100 μL of 6μg/ml of AF 488-conjugated human CD70 Protein (Site-Specific AF 488 Conjugation, ECD, His Tag, Cat. No. 10780-H85H5-SD) and negative control protein. Non-transduced 293 cells were used as a control (left). AF 488 signal was used to evaluate the binding activity (QC tested). 2.Binding activity of AF 488-conjugated CD70 protein to PBMC cells. PBMC cells were stained with PE conjugated anti-CD3 antibody and AF 488-conjugated human CD70 Protein (Site-Specific AF 488 Conjugation, ECD, His Tag, Cat. No. 10780-H85H5-SD) and detected by flow cytometry. PE signal was used to evaluate the content of CD3+ T cells in PBMCs. AF 488 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Gly
蛋白分子量：The protein has a predicted molecular mass of 54.8 kDa.
蛋白NP号：NP_001243.1
蛋白氨基酸序列：Gln39-Pro193
蛋白名称：Human CD70/CD27 Ligand Protein (Site-Specific AF 488 Conjugation, ECD, His Tag)
蛋白构建：A DNA sequence encoding the human CD70 (NP_001243.1) (Gln 39-Pro 193) was expressed with a N-terminal polyhistidine tag. This construct has a trimer design.The protein is site-specifically conjugated with AF 488 (Excitation Max.= 495 nm, Emission Max.= 519 nm).
表达宿主：HEK293 Cells
蛋白纯度：≥ 90 % as determined by SDS-PAGE.
蛋白活性：1.Flow cytometric analysis of anti-CD70 CAR expression. 5e5 of anti-CD70 CAR-293 cells were stained with 100 μL of 6μg/ml of AF 488-conjugated human CD70 Protein (Site-Specific AF 488 Conjugation, ECD, His Tag, Cat. No. 10780-H85H5-SD) and negative control protein. Non-transduced 293 cells were used as a control (left). AF 488 signal was used to evaluate the binding activity (QC tested). 2.Binding activity of AF 488-conjugated CD70 protein to PBMC cells. PBMC cells were stained with PE conjugated anti-CD3 antibody and AF 488-conjugated human CD70 Protein (Site-Specific AF 488 Conjugation, ECD, His Tag, Cat. No. 10780-H85H5-SD) and detected by flow cytometry. PE signal was used to evaluate the content of CD3+ T cells in PBMCs. AF 488 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Gly
蛋白分子量：The protein has a predicted molecular mass of 54.8 kDa.
蛋白NP号：NP_001243.1
蛋白氨基酸序列：Gln39-Pro193
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重组人CD70/CD27 Ligand蛋白(AF 488-conjugated)
￥3480 - 11180
