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文献和实验Quantification of Human IgG and Related Fc Fusion Proteins by a Human IgG/Fc Capture ELISA
of recombinant human IgGs after production and purification for further assays. Here, we describe a human IgG/Fc capture enzyme linked immunosorbent assay (ELISA) which is simple, robust, inexpensive and specific for all types of human IgGs and related Fc fusion
in immunotherapy because of their highly restricted fine specificity. Expression of specific peptides in complex with MHC class I molecules on cells was shown to be associated with cancer and autoimmune disorders (1 –8 ). For example, human melanomas express
Purification Strategy for Recombinant Formsof the Human Mitochondrial DNA Helicase
In this chapter, we present a streamlined purification for the production of near-homogeneous and high yield recombinant forms of the human mitochondrial DNA helicase. Minimizing the number of steps and the time elapsed for purification
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