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- 详细信息
- 文献和实验
- 技术资料
- 库存:
现询
- 英文名:
L-Pyroglutamic acid(10mM in Water)
- CAS号:
98-79-3
- 保质期:
Store at -20℃,6 months.
- 供应商:
北京索莱宝科技有限公司
- 保存条件:
Store at -20℃,6 months.
- 规格:
1ml
L-Pyroglutamic acid 是 Pyroglutamic acid 的左旋异构体。

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文献和实验Metabolic labeling Immunoprecipitation
/ml Cysteine-HCl,100µg/ml methionine,sterile filtered • Tris-buffered saline (TBS): 20mM Tris,150mM NaCl,pH 7.4 • X1000 Protease inhibitor soln: 500mM PMSF (Sigma P-7626)in EtOH,10mg/ml leupeptin (Sigma L-2023)in water,10mg/ml pepstatin A (P-4265
Metabolic labeling Immunoprecipitation
P-7626) in EtOH, 10mg/ml leupeptin (Sigma L-2023) in water, 10mg/ml pepstatin A (P-4265) in dimethylformamide • IP buffer: 1% Triton x-100, 0.05% NP-40 in TBS • x2 IP buffer: 2% Triton x-100, 0.1% NP-40 in TBS • ProteinG agarose (GIBCO #15920
Farnham Lab Chromatin Immunoprecipitation (ChIPs) Protocol for Tissues
. You can also include a "mock" sample which contains 1X dialysis buffer instead of chromatin (IgG and mock are critical to control for nonspecific interactions and DNA contamination of IP and wash solutions). Remove 10% of the amount of chromatin used for one IP
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