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- Hello Bio
- 英国
- HB0787
- 2026年03月22日
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- 技术资料
- 保存条件：
-20°C
- 英文名：
Hoechst 33342
- 供应商：
Hello Bio
- CAS号：
875756-97-1
- 规格：
50mg///100mg
Overview
Blue fluorescent DNA stain that is commonly used in fluorescent microscopy. It is frequently used as a nuclear stain to stain nuclei. It is excited by UV light.
Hoechst 33342 is cell permeable and has greater cell permeability than Hoechst 33258. The stain can be used on both live and fixed cells and is often used as an alternative to DAPI.
Hoechst 33342 binds to the AT-rich regions of the minor grove in DNA which renders it specific for nuclear chromatin. Its fluorescent intensity depends on the DNA content, chromatin structure and the position of the cell within the cell cycle.
Uses and applications
There is little fluorescent overlap with other commonly used small-molecule fluorophores / fluorescent proteins that emit in the green / red range. This makes it suitable for a wide range of applications.
Counterstain
Hoechst 33342 is commonly used as a counterstain in fluorescent imaging.
Cell cycle studies / Apoptosis
Hoechst 33342 can stain the condensed nuclei of apoptotic cells to allow the identification of chromatin condensation and fragmentation. It is commonly used with propidium iodide to distinguish normal/live apoptotic and dead cell populations. It can additionally be used in conjunction with arcidine orange (AO) to distinguish apoptotic cells.
Incorporation of Brdu into DNA has a quenching effect on Hoechst fluorescence. Hoechst 33342 is also used in combination with BrdU to monitor cell cycle progression.
Stem cells
Combination of the Hoechst 33342 stain with surface-marker phenotyping allows the characterisation of a sub-population of stem cells termed the ‘side population’ (SP).
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文献和实验Isolation of Resident Cardiac Progenitor Cells by Hoechst 33342 Staining
of stem cells to efficiently efflux vital dyes such as Hoechst 33342. During fluoresence activated cell sorting (FACS) such Hoechst-extruding cells appear to the side of Hoechst-dye retaining cells and have thus been termed side population (SP) cells
1．悬浮生长的细胞在培养状态下加入Heochst 33342，终浓度为1μg/ml；帖壁生长的细胞用含有0.02％EDTA的0.25％胰蛋白酶消化成单细胞悬液，离心，弃上清，用1ml全培养液重悬细胞，加入Heochst 33342，终浓度为1μg/ml，37℃孵育7～10min。 2．4℃ 500～1000r/min离心弃去染液。 3．加入1.0ml PI染液，4℃避光染色15min。 4．400目的筛网过滤1次。 5．流式细胞仪分析。
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