DNA-Free HSTaq-D UDG qPCR 2× S

DNA-Free HSTaq-D UDG qPCR 2× SuperMix (SYBR Green) is used for the quantitative analysis of DNA molecules. This product contains hot-start Taq-D DNA polymerase, UDG, dNTPs, dUTP, SYBR Green, and an optimized reaction buffer at a 2× concentration. For qPCR, simply add the template, primers, and water to adjust the 2× SuperMix solution to a 1× concentration for the reaction. Sample types include genomic DNA, plasmid DNA, phage DNA, cDNA, and more. The hot-start Taq-D DNA polymerase is an antibody-modified engineered KlenTaq enzyme without 5’ exonuclease activity, offering strong specificity and high detection sensitivity. The buffer is specifically optimized for qPCR, making it suitable for high-specificity and high-sensitivity qPCR detection. The inclusion of UDG and dUTP prevents aerosol cross-contamination of samples. Some qPCR instrument models may require ROX reference dye.

Cat. No.: FHTDUMG-1 (for 1ml)

Cat. No.: FHTDUMG-5 (for 5ml)

Cat. No.: FHTDUMG-20 (for 20ml)

Cat. No.: FHTDUMG-50 (for 50ml)

Description

DNA-Free HSTaq-D UDG qPCR 2× SuperMix (SYBR Green) is used for the quantitative analysis of DNA molecules. This product contains hot-start Taq-D DNA polymerase, UDG, dNTPs, dUTP, SYBR Green, and an optimized reaction buffer at a 2× concentration. For qPCR, simply add the template, primers, and water to adjust the 2× SuperMix solution to a 1× concentration for the reaction. Sample types include genomic DNA, plasmid DNA, phage DNA, cDNA, and more. The hot-start Taq-D DNA polymerase is an antibody-modified engineered KlenTaq enzyme without 5’ exonuclease activity, offering strong specificity and high detection sensitivity. The buffer is specifically optimized for qPCR, making it suitable for high-specificity and high-sensitivity qPCR detection. The inclusion of UDG and dUTP prevents aerosol cross-contamination of samples. Some qPCR instrument models may require ROX reference dye.

Features

• Completely free from bacterial DNA.
• The inclusion of UDG and dUTP prevents aerosol cross-contamination of samples.
• Reduces qPCR operation time.
• Prevents contamination from multiple steps.
• Quantification of DNA fragments (≤3 kb).
• Hot-start Taq-D DNA polymerase reduces false positive signals due to non-specific amplification.
• High tolerance to PCR inhibitors, suitable for direct qPCR.

Applications

• SYBR Green dye-based qPCR detection.