DNA-Free HSTaq UDG qPCR 2× Sup

Cat. No.: FHTUMG-1 (for 1ml)

Cat. No.: FHTUMG-5 (for 5ml)

Cat. No.: FHTUMG-20 (for 20ml)

Cat. No.: FHTUMG-50 (for 50ml)

Description

DNA-Free HSTaq UDG qPCR 2× SuperMix (SYBR Green) is a specialized premix for qPCR reactions using the SYBR Green I intercalation method. It can be used for the quantitative analysis of double-stranded DNA and single-stranded cDNA molecules from sample types such as genomic DNA, plasmid DNA, phage DNA, cDNA, and more. This product contains hot-start Taq DNA polymerase, UDG, dNTPs, dUTP, SYBR Green, and an optimized reaction buffer at a 2× concentration. For qPCR, simply add the template, primers, and water to adjust the 2× SuperMix solution to a 1× concentration for the reaction. The core component, hot-start Taq DNA polymerase, is an antibody-modified engineered Taq enzyme, offering strong specificity, high detection sensitivity, and high amplification yield. The buffer is specifically optimized for qPCR, making it suitable for high-specificity and high-sensitivity qPCR detection. The inclusion of UDG and dUTP prevents aerosol cross-contamination of samples. Some qPCR instrument models may require ROX reference dye.

Features

• Completely free from bacterial DNA.
• Reduces qPCR operation time.
• Prevents contamination from multiple steps.
• Quantification of DNA fragments (≤4 kb).
• Hot-start Taq enzyme reduces false positive signals due to non-specific amplification.
• High amplification efficiency and good stability.

Applications

• SYBR Green dye-based qPCR detection.