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- SBS
- DFMC2-2k (for 2KU)
- 2026年04月08日
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DNA-Free mRNA Cap 2'-O-Methyltransferase adds a methyl group to the 2'-OH of the first nucleotide adjacent to the 5´ cap structure of RNA. Using S-adenosyl methionine (SAM) as a methyl donor, it methylates capped RNA to form Cap-1. Studies have shown that the 2'-O-methylation in the Cap-1 structure can help mRNA evade immune responses in certain eukaryotic cells.
Cat. No.: DFMC2-2k (for 2KU)
Cat. No.: DFMC2-10k (for 10KU)
Description
DNA-Free mRNA Cap 2'-O-Methyltransferase adds a methyl group to the 2'-OH of the first nucleotide adjacent to the 5´ cap structure of RNA. Using S-adenosyl methionine (SAM) as a methyl donor, it methylates capped RNA to form Cap-1. Studies have shown that the 2'-O-methylation in the Cap-1 structure can help mRNA evade immune responses in certain eukaryotic cells.
mRNA Cap 2'-O-Methyltransferase specifically methylates RNA with an m7GpppN cap structure at the 5’ end but does not methylate RNA with 5’ ends of pN, ppN, pppN, or GpppN. RNA with an m7GpppN cap structure can be obtained by adding cap analogs during in vitro transcription or using an in vitro capping system. 2 kU of mRNA Cap 2'-O-Methyltransferase can methylate 400 μg of capped RNA.
Our DNA-Free enzymes are all recombinant proteins and purified through multiple steps, devoid of genomic and plasmid DNA from the production strain.
Unit Definition
One unit is defined as the amount of enzyme required to methylate 10 pmol of an 80 nt capped RNA transcript within 60 minutes at 37℃ under 1× mRNA Cap 2'-O-Methyltransferase reaction buffer conditions.
Features
- Completely free from bacterial DNA.
- Improves mRNA expression after microinjection and transfection.
- Enhances RNA translation efficiency.
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文献和实验mRNA的帽状结构 cap structure（of mRNA）
XMe 。 第二个核苷也甲基化，是最普通的帽状结构。第二个核苷（ x）多为腺嘌呤核苷，也有鸟嘌呤核苷或嘧啶核苷。这些核苷的甲基化一定是在 2′ -核酸的位置上，腺嘌呤核苷的腺嘌呤的 6位也可被甲基化，形成二甲基（ 2′－ O， N6 ）腺嘌呤核苷：（ 3） Cap2： 7Me Gppp XMe p YMe ，为 7-甲基鸟嘌呤核苷加上第二个核苷（ x）的甲基化，第三个核苷（ Y）的核糖（ 2′－ C）也甲基化。这种结构在动物细胞中均有微量存在。帽状结构，由 RNA的 5′末端
of containing most of the necessary reagents pre-packaged in RNase-free quality （e.g. polyATtract from Promega or Oligotex mRNA kit from Qiagen work well for Neurospora, Table 1）。 Depending on the kit size, this allows isolation of poly（A）-RNA from 0.25 to 5 mg
低至单个细胞的DNA&mRNA共提取方案，起始样本支持显微切割细胞、流式分选细胞、循环肿瘤细胞（CTCs）、胎儿细胞、干细胞、T细胞等。
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