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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Anti-I kappa B-alpha (Phospho-Tyr305) Antibody
- 抗体英文名:
Anti-I kappa B-alpha (Phospho-Tyr305) Antibody
- 浓度:
/
- 应用范围:
Western Blotting, IHC
- 宿主:
Rabbit
- 克隆性:
Polyclonal
- 保存条件:
-20°C
- 亚型:
/
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文献和实验In situ hybridisation to alpha satellite sequences (chromosome specific)
solution and replace with mouse anti-biotin antibody diluted 1:100 in blocking solution for 40 minutes at 37C. We currently use DAKO antibody #M0743, 100-200m l per slide. (10) Wash with blocking solution for 10 minutes (11) Incubate with rabbit
. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.
Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
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