- ￥3480
- LSMBIO
- 1.56-100ng/ml
- 0.78ng/ml
- 进口
- ELA-E0452b
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
1.56-100ng/ml
- 检测方法：
夹心法
- 应用：
ELISA方法检测牛血清、血浆或其它相关生物液体中天然和重组蛋白的含量
- 适应物种：
牛
- 样本：
牛血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.78ng/ml
- 规格：
96tests
Bovine Thyroxine,T4 ELISA Kit
Product Name:Bovine Thyroxine,T4 ELISA Kit
Packing:96T
Catalog No.:Bovine Thyroxine,T4 ELISA Kit
Gene Name:Bovine Thyroxine,T4
Detect Range:1.56-100ng/mL
Sensitivity:0.78ng/mL
Target Protein Name:Bovine Thyroxine,T4
Alternative Name:Bovine Thyroxine,T4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Thyroxine,T4 ELISA Kit allows for the in vitro quantitative determination of Bovine Thyroxine,T4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Thyroxine,T4 ELISA Kit has been pre-coated with an Bovine Thyroxine,T4 antibody specific to Bovine Thyroxine,T4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Thyroxine,T4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Thyroxine,T4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Thyroxine,T4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
TUNEL PROCEDURE IN BOVINE EMBRYOS
. Dilute 1 µl RNase A (stock) with 999 µl PBS/PVP and then dilute this solution 1:1 with PBS/PVP to obtain a working solution (Use fresh). Mounting medium and antifade: ProLong Antifade Kit (Molecular
【交流】关于NEB的T4 DNA连接酶和快速连接试剂盒的常见问题及解答
连接酶，反应10分钟。平滑末端连接：在20µl反应体系中加入1µl T4 DNA 连接酶反应2小时，或者加入1µl高浓度T4 DNA 连接酶反应10分钟。 另外，NEB的快速连接试剂盒 (Quick Ligation Kit, NEB #M2200V [15个反应])是独有的可以在室温5分钟条件下连接平滑末端和粘性末端的试剂盒。 图1：在25°C条件下，用1 unit（1:400稀释后取1 µl）T4 DNA连接酶连接λDNA/Hind Ⅲ片段(4-碱基粘端)不同反应时间的结果。 图
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