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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白的浓度
- 适应物种:
大鼠
- 标记物:
Rho/ Rat Rho
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.78pg/ml
- 规格:
96tests
Rat Rho ELISA试剂盒
Product Name:Rat Rho ELISA试剂盒
Packing:96T
Catalog No.:Rat Rho ELISA试剂盒
Gene Name:Rat Rho
Detect Range:78-5000pg/mL
Sensitivity:46.79pg/mL
Target Protein Name:Rat Rho
Alternative Name:Rat Rho
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Rat Rho ELISA KIT allows for the in vitro quantitative determination of Rat Rho concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Rat Rho ELISA KIT has been pre-coated with an Rat Rho antibody specific to Rat Rho .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Rat Rho and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rat Rho, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Rat Rho in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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RAT/MOUSE GROWTH HORMONE ELISA KIT
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【求助】有使用过试剂盒检测Rho活性的同仁吗?快来指导下我~~
adria 试剂盒才买回来,是upstate的那个,rho activation assay kit研读说明书中,我以前从没做过pull down assay的,同实验室也没师兄师姐做过...所以,基本等于小白一个 问题是这样的,在rho pull down assay的这个步骤中,最后要求短暂离心收集管底物质。在免疫分析这一步又要求讲上清和琼脂糖珠混合,把我弄糊涂了,那前一步收集的管底物质是干什么的? 试剂盒很贵,实在不敢浪费,请
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