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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测兔血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
兔
- 标记物:
Rabbit Elongation factor 1-alpha 1,EEF1A1
- 样本:
兔血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rabbit EEF1A1 ELISA KIT
Product Name:Rabbit EEF1A1 ELISA KIT
Packing:96T
Catalog No.:Rabbit EEF1A1 ELISA KIT
Gene Name:Rabbit EEF1A1
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Rabbit EEF1A1Rabbit EEF1A1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Rabbit EEF1A1 ELISA KITRabbit EEF1A1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Rabbit EEF1A1 ELISA KIT has been pre-coated with an Rabbit EEF1A1 antibody specific to Rabbit EEF1A1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Rabbit EEF1A1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rabbit EEF1A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Rabbit EEF1A1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验High‐Throughput, Multiplexed Analysis of 3‐Nitrotyrosine in Individual Proteins
. In this paper, we describe our antibody microarray protocol for detecting 3?nitrotyrosine in specific proteins. Curr. Protoc. Toxicol. 51:17.15.1?17.15.16. © 2012 by John Wiley & Sons, Inc. Keywords: 3?nitrotyrosine; ELISA microarray
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