- ￥3480
- LSMBIO
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- CELI-66039p
- 2026年05月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSMBIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测猪血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
猪
- 标记物：
Porcine Asymmetric dimethylarginine,ADMA
- 样本：
猪血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Porcine asymmetrical dimethylarginine, ADMA ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Asymmetric dimethylarginine, ADMA
Search name
Porcine ADMA ELISA Kit ,Porcine asymmetrical dimethylarginine ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of asymmetric dimethylarginine,Asymmetric dimethylarginine
concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Asymmetric dimethylarginine, During the reaction, Asymmetric dimethylarginine in the sample or
standard competes with a fixed amount of biotin-labeled Asymmetric dimethylarginine for sites on a pre-coated Monoclonal antibody
specific to Asymmetric dimethylarginine. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Asymmetric dimethylarginine in the samples is then
determined by comparing the O.D. of the samples to the standard curve.
Porcine-asymmetrical-dimethylarginine-ADMA-ELISA-Kit
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文献和实验Measurement of Arginine Metabolites: Regulators of Nitric Oxide Metabolism
., Wang, J., and Wang, X.L. 2007. Asymmetric dimethylarginine (ADMA) in vascular, renal and hepatic disease and the regulatory role of L‐arginine on its metabolism. Mol. Genet. Metab. 91:309‐317.
： PI和Hoechst33342都是sigma公司的粉剂，不是kit，2、具体方法： 将PI和Hoechst33342的终浓度调到10μg/mg就行，用PBS，Hank's液、生理盐水或D液任何一种溶解都可以。Hoechst33342染色37℃10min就达到饱和，PI在4℃10~20min就行。3、文献出处： 《细胞实验指南》上册，p102－125，现代生物技术译从－－科学出版社， 有好几篇文献，只列出其中的一个： Ha HC, Snyder SH. Poly(ADP-ribose
进口 F6436 Porcine Insulin ELISA* ELISA 96T 3500 原装进口 F6437 Rat High Range Insulin* ELISA 96T 3500
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