Rabbit CACNA1E ELISA KIT

Rabbit Voltage- dependent R- type calcium channel subunit alpha- 1E, CACNA1E ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

CACNA1E,BII; CACH6; CACNL1A6; Cav2.3; brain calcium channel II; calcium channel, L type, alpha-1 polypeptide; calcium channel, R type, alpha-1 polypeptide; calcium channel, voltage-dependent, alpha 1E subunit; voltage-dependent R-type calcium channel subunit alpha-1E; voltage-dependent calcium channel alpha 1E subunit; voltage-gated calcium channel alpha 1E subunit; voltage-gated calcium channel alpha subunit Cav2.3; voltage-gated calcium channel subunit alpha Cav2.3; calcium channel, voltage-dependent, R type, alpha 1E subunit

Search name

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Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rabbit Voltage- dependent R- type calcium channel subunit alpha- 1E, CACNA1E concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to CACNA1E. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CACNA1E and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CACNA1E, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CACNA1E in the samples is then determined by comparing the O.D. of the samples to the standard curve.