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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测兔血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
兔
- 标记物:
Rabbit Cytochrome P450 19A1,CYP19A1
- 样本:
兔血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rabbit Cytochrome P450 19A1, CYP19A1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Cyp19a1,aromatase,ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM; cytochrome P-450AROM; cytochrome P450 19A1; cytochrome P450, subfamily XIX (aromatization of androgens); estrogen synthase; estrogen synthetase; flavoprotein-linked monooxygenase; microsomal monooxygenase; cytochrome P450 family 19 subfamily A polypeptide 1
Search name
Rabbit Cyp19a1 ELISA KIT ,Rabbit aromatase ELISA KIT ,Rabbit ARO ELISA KIT ,Rabbit ARO1 ELISA KIT ,Rabbit CPV1 ELISA KIT ,Rabbit CYAR ELISA KIT ,Rabbit CYP19 ELISA KIT ,Rabbit CYPXIX ELISA KIT ,Rabbit P-450AROM ELISA KIT ,Rabbit cytochrome P-450AROM ELISA KIT ,Rabbit cytochrome P450 19A1 ELISA KIT ,Rabbit cytochrome P450 ELISA KIT ,Rabbit subfamily XIX (aromatization of androgens) ELISA KIT ,Rabbit estrogen synthase ELISA KIT ,Rabbit estrogen synthetase ELISA KIT ,Rabbit flavoprotein-linked monooxygenase ELISA KIT ,Rabbit microsomal monooxygenase ELISA KIT ,Rabbit cytochrome P450 family 19 subfamily A polypeptide 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rabbit Cytochrome P450 19A1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 19A1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cytochrome P450 19A1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cytochrome P450 19A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cytochrome P450 19A1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Isolation and Purification of Constitutive Forms of Microsomal Cytochrome P450
from microsomes through the use of proteases were not successful, resulting merely in the solubilization of a number of other microsomal proteins. The breakthrough event was the solubrlizatron of rabbit liver microsomal cytochrome P450 using the ionic detergent
小鼠细胞色素P450(Cytochrome P450)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 小鼠细胞色素P450 ( Cytochrome P450 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗小鼠 Cytochrome P450 单抗包被于酶标板上,标准品和样品中的 Cytochrome P450与单抗结合,加入生物素化的抗小鼠
Expression of Eukaryotic Cytochromes P450 in E. coli
Although much has been learned from the study of relatively abundant cytochromes P450 (e.g., P450cam from Pseudomonas putida cytosol, P4501A2 and P4502B4 from rabbit liver microsomes, and P45Oscc from bovine adrenal cortex mitochondria
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