- ￥3480
- LSMBIO
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-02117Ra
- 2025年07月22日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测兔血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
兔
- 标记物：
Rabbit Pyruvate kinase isozymes M1/M2,PKM2
- 样本：
兔血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rabbit Pyruvate kinase isozymes M1/M2, PKM2 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
M2- PK,PKM,CTHBP,HEL-S-30,OIP3,PK3,PKM2,TCB,THBP1,OIP-3,OPA-interacting protein 3,PK muscle type,cytosolic thyroid hormone-binding protein,epididymis secretory protein Li 30,p58,pyruvate kinase 2/3,pyruvate kinase PKM,pyruvate kinase isozymes M1/M2,pyruvate kinase muscle isozyme,thyroid hormone-binding protein 1,thyroid hormone-binding protein cytosolic,tumor M2-PK,pyruvate kinase muscle,Tumor M2-PK,Thyroid hormone-binding protein 1,THBP1,Pyruvate kinase muscle isozyme,Pyruvate kinase 2/3,p58,Cytosolic thyroid hormone-binding protein,CTHBP,Opa-interacting protein 3
Search name
Rabbit M2- PK ELISA KIT ,Rabbit M2PK ELISA KIT,Rabbit PKM ELISA KIT ,Rabbit CTHBP ELISA KIT ,Rabbit HEL-S-30 ELISA KIT ,Rabbit OIP3 ELISA KIT ,Rabbit PK3 ELISA KIT ,Rabbit PKM2 ELISA KIT ,Rabbit TCB ELISA KIT ,Rabbit THBP1 ELISA KIT ,Rabbit OIP-3 ELISA KIT ,Rabbit OPA-interacting protein 3 ELISA KIT ,Rabbit PK muscle type ELISA KIT ,Rabbit cytosolic thyroid hormone-binding protein ELISA KIT ,Rabbit epididymis secretory protein Li 30 ELISA KIT ,Rabbit p58 ELISA KIT ,Rabbit pyruvate kinase 2/3 ELISA KIT ,Rabbit pyruvate kinase PKM ELISA KIT ,Rabbit pyruvate kinase isozymes M1/M2 ELISA KIT ,Rabbit pyruvate kinase muscle isozyme ELISA KIT ,Rabbit thyroid hormone-binding protein 1 ELISA KIT ,Rabbit thyroid hormone-binding protein cytosolic ELISA KIT ,Rabbit tumor M2-PK ELISA KIT ,Rabbit pyruvate kinase muscle ELISA KIT ,Rabbit Tumor M2-PK ELISA KIT ,Rabbit Thyroid hormone-binding protein 1 ELISA KIT ,Rabbit THBP1 ELISA KIT ,Rabbit Pyruvate kinase muscle isozyme ELISA KIT ,Rabbit Pyruvate kinase 2/3 ELISA KIT ,Rabbit p58 ELISA KIT ,Rabbit Cytosolic thyroid hormone-binding protein ELISA KIT ,Rabbit CTHBP ELISA KIT ,Rabbit Opa-interacting protein 3 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rabbit Pyruvate kinase isozymes M1/M2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate kinase isozymes M1/M2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Pyruvate kinase isozymes M1/M2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Pyruvate kinase isozymes M1/M2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Pyruvate kinase isozymes M1/M2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Steady State ATPase Assays Coupled Enzyme System
) can be plotted vs MT concentration and the data points fit with the Michaelis-Menton equation using a program such as Kaleidagraph. The equation for the curve fit where y= kcat and x=[MTs] is: y = m1 * x / (m2 + x) m1
secondary antibody review -- data from 99 publications
western blot test Benzonase activity in PMCA reactions containing biotin-labeled oligo(dT), 17 goat IgG FITC flow cytometry 1:250 Jackson ImmunoResearch Laboratories 17 goat AP ELISA 1:1000 18 HRP western blot Cell
【资源】全面归类网址：毕赤酵母表达各种来源蛋白（细菌，真菌，植物，人类等）
2, 320 IU/L, active531BP230 antigenI, 480 ug/L546Bile salt stimulated lipaseS, 1 g/L, native, INV[207], 521Bivalent diabody against carcinoembryonic antigen (CEA), T-cell coreceptor CD3-cell coreceptor CD2S, 1 mg/L, α-MF[208]c-kit receptor kinase domainI
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