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文献和实验In this chapter, we describe a method for detecting the ubiquitination status of G protein-coupled receptors (GPCRs). This involves co-expression of a GPCR with an epitope-tagged ubiquitin construct in a �heterologous mammalian expression
that for several proteins, the first ubiquitin moiety is fused linearly to the α-NH2 group of the N-terminal residue. Important biological questions relate (1) to the evolutionary requirement for an alternative mode of ubiquitination, (2) to the identity of the set
Ubiquitinated protein detection is often troublesome since in most cases this modification reduces the half-life of targeted proteins, inducing their degradation. Furthermore, ubiquitination is reversible thanks to the action of highly
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