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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
T7opt in pCAGGS (T7 RNA polymerase)
- 库存：
100
- 供应商：
LSMBIO
- 规格：
2ug
T7opt in pCAGGS(T7 RNA polymerase)
Catalog No. PVT10506
Packing 2ug
Function Protease plasmids
Resistance Amp
Screen /
Strain DH5alpha
Culture temperature 37degrees centigrade
Replicon pUC
Copy high
Promoter CAG
Induction --
Forward primer pCAGGS-F
Reverse primer
T7opt in pCAGGS(T7 RNA polymerase) information
Promoter: CAG
Replicon: pUC
Prokaryotic resistance: ampicillin Amp
Cloned strain: Escherichia coli DH5 alpha
Culture conditions: 37 degrees centigrade
T7opt in pCAGGS(T7 RNA polymerase) Description
T7opt in pCAGGS (T7 RNA polymerase) is a T7 RNA polymerase expression plasmid.
T7opt in pCAGGS(T7 RNA polymerase) Sequence
LOCUS Exported 7381 bp ds-DNA circular SYN 26-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..7381
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 1725..4376
/codon_start=1
/label=T7 RNA polymerase
/translation="MNTINIAKNDFSDIELAAIPFNTLADHYGERLAREQLALEHESYE
MGEARFRKMFERQLKAGEVADNAAAKPLITTLLPKMIARINDWFEEVKAKRGKRPTAFQ
FLQEIKPEAVAYITIKTTLACLTSADNTTVQAVASAIGRAIEDEARFGRIRDLEAKHFK
KNVEEQLNKRVGHVYKKAFMQVVEADMLSKGLLGGEAWSSWHKEDSIHVGVRCIEMLIE
STGMVSLHRQNAGVVGQDSETIELAPEYAEAIATRAGALAGISPMFQPCVVPPKPWTGI
TGGGYWANGRRPLALVRTHSKKALMRYEDVYMPEVYKAINIAQNTAWKINKKVLAVANV
ITKWKHCPVEDIPAIEREELPMKPEDIDMNPEALTAWKRAAAAVYRKDKARKSRRISLE
FMLEQANKFANHKAIWFPYNMDWRGRVYAVSMFNPQGNDMTKGLLTLAKGKPIGKEGYY
WLKIHGANCAGVDKVPFPERIKFIEENHENIMACAKSPLENTWWAEQDSPFCFLAFCFE
YAGVQHHGLSYNCSLPLAFDGSCSGIQHFSAMLRDEVGGRAVNLLPSETVQDIYGIVAK
KVNEILQADAINGTDNEVVTVTDENTGEISEKVKLGTKALAGQWLAYGVTRSVTKRSVM
TLAYGSKEFGFRQQVLEDTIQPAIDSGKGLMFTQPNQAAGYMAKLIWESVSVTVVAAVE
AMNWLKSAAKLLAAEVKDKKTGEILRKRCAVHWVTPDGFPVWQEYKKPIQTRLNLMFLG
QFRLQPTINTNKDSEIDAHKQESGIAPNFVHSQDGSHLRKTVVWAHEKYGIESFALIHD
SFGTIPADAANLFKAVRETMVDTYESCDVLADFYDQFADQLHESQLDKMPALPAKGNLN
LRDILESDFAFA"
polyA_site 4450..4505
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal"
promoter 5039..5234
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 5085..5220
/label=SV40 ori
/note="SV40 origin of replication"
polyA_signal 5240..5374
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(5613..6201)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6372..7232)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(7233..7337)
/gene="bla"
/label=AmpR promoter
misc_feature 7368..1664
/label=CAG promoter
ORIGIN
1 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
361 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
421 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
481 gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg
541 agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg
601 cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcggggag tcgctgcgac
661 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
721 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
781 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
841 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
901 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
961 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
1021 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
1081 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
1141 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
1201 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
1261 ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
1321 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
1381 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
1441 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
1501 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
1561 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
1621 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
1681 tggttattgt gctgtctcat cattttggca aagaattcgc caccatgaac accatcaata
1741 ttgccaagaa cgacttttct gatatcgagc tggccgctat tccattcaat acactggctg
1801 accactacgg agagcggctg gcccgcgaac agctggctct ggagcatgaa agctatgaga
1861 tgggagaagc ccgattcagg aagatgtttg agaggcagct gaaagctggg gaagtggcag
1921 acaacgcagc cgctaagcca ctgattacca cactgctgcc caaaatgatc gccagaatta
1981 atgattggtt cgaggaagtg aaggcaaaac gaggaaagag gcctaccgcc ttccagtttc
2041 tgcaggagat caagccagaa gctgtggcat acatcaccat caagactacc ctggcatgcc
2101 tgacaagcgc cgacaacaca actgtgcagg cagtcgcttc cgcaatcgga cgagctattg
2161 aggacgaagc acgctttggg agaatccggg atctggaggc caagcacttc aagaaaaacg
2221 tggaggaaca gctgaacaag agggtggggc atgtctataa gaaggccttc atgcaggtgg
2281 tcgaggccga catgctgtca aagggactgc tgggaggaga ggcctggagc tcctggcaca
2341 aagaagatag catccatgtg ggagtccgct gcatcgagat gctgattgaa tctactggga
2401 tggtgagtct gcaccgacag aacgccggcg tggtcggaca ggactctgag acaatcgaac
2461 tggctcccga gtatgccgaa gctattgcaa ctagagccgg agctctggca gggatcagtc
2521 caatgttcca gccctgcgtg gtccccccta agccttggac tggcatcacc gggggcggat
2581 actgggctaa tggaaggaga ccactggcac tggtgcgaac acactctaag aaagccctga
2641 tgagatacga ggatgtctat atgcccgaag tgtataaggc catcaacatt gctcagaata
2701 cagcatggaa aattaacaag aaagtgctgg ccgtcgctaa tgtgatcact aagtggaaac
2761 attgtcccgt ggaggacatc cctgccattg aacgggagga actgcctatg aagccagagg
2821 acatcgatat gaaccctgaa gctctgaccg catggaagcg cgcagccgct gcagtgtaca
2881 gaaaggataa agcccggaag tcccggcgca tttctctgga gttcatgctg gaacaggcca
2941 acaagtttgc taatcacaaa gcaatctggt tcccctacaa catggactgg cgcggacgag
3001 tctatgccgt gagcatgttc aaccctcagg ggaatgatat gacaaagggc ctgctgactc
3061 tggctaaggg gaaaccaatt gggaaggagg gctactattg gctgaaaatc cacggggcca
3121 attgcgctgg cgtcgacaag gtgccattcc ccgagaggat caagttcatc gaggaaaacc
3181 atgaaaatat tatggcatgt gccaagtctc ccctggagaa cacatggtgg gccgaacagg
3241 atagtccttt ctgctttctg gccttctgtt ttgagtacgc tggagtgcag caccatgggc
3301 tgagttataa ttgctccctg ccactggcct ttgacggctc ttgtagtgga atccagcact
3361 tctccgcaat gctgagggat gaggtcggag gaagagcagt gaacctgctg ccatctgaga
3421 cagtgcagga catctacggc attgtcgcca agaaagtgaa tgagatcctg caggctgacg
3481 caattaacgg gact
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文献和实验该产品被引用文献
Upregulation of Antioxidant Capacity and Nucleotide Precursor Availability Suffices for Oncogenic Transformation
Author：YangZhang1YiXu1WenyunLu23Jonathan M.Ghergurovich24LiliGuo56Ian A.Blair5Joshua D.Rabinowitz23XiaoluYang17
Received 22 January 2020, Revised 4 August 2020, Accepted 30 September 2020, Available online 6 November 2020.
Published: November 6, 2020
Cell Metabolism
Available online 6 November 2020 In Press
doi: doi.org/10.1016/j.cmet.2020.10.002
相关实验
mRNA Amplification with T7 RNA Polymerase
on a 42ºC oven and set PCR machine to hold at 70ºC. In an Eppendorf tube add: 1 µg Total RNA 1 µL T7 oligo(dT) primer q.s. to 12 µL with Nuclease-free H2 O Incubate 10 min. at 70ºC. Spin briefly to pull
Oocyte Expression With Injection of Purified T7 RNA Polymerase
of complementary DNA (cDNA). We report on a third expression technique that is based on the combined injection of cDNA and purified T7 RNA polymerase directly into the cytoplasm of oocytes.
T7 RNA Polymerase-Mediated Incorporation of 8-N3AMP Into RNA for Studying Protein-RNA Interactions
Ultraviolet (UV)-dependent photochemical crosslinking is a powerful approach that can be used for the identification of RNA-protein interactions. Although 8-azidoATP (8-N3 ATP) has been widely used to elucidate the ATP binding site
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T7opt in pCAGGS (T7 RNA polymerase)
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