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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
Immunotag™ Rat TYRO protein tyrosine kinase-binding protein ELISA Kit
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
96wellplate
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文献和实验Immunoprecipitation of PDE2 Phosphorylated and Inactivated by an Associated Protein Kinase
4 . I describe protocols for producing an antibody to an aminoterminal bacterial fusion protein encoding amino acids 1–251 of PDE2A2 as well as the use of this antibody in immunoprecipitating a PDE2:tyrosine protein-kinase complex from rat brain or PC12 cells.
Purpose and backgrounds Principle of radioiodination Addition of oxidizing reagents (such as chloramine-T or peroxidase+H2 02 ) converts I- to I+ or I3- . This highly reactive molecule attacks o-position of tyrosine (or in some case
our extensive experience with B-PER for isolating fusion proteins in which the fusion partner is either IPIII-His (an internal protein of bacteriophage T4 with a His tag at the C-terminus)or MBP (the E.coli maltose binding protein).The two cases illustrate
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