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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
Immunotag™ Chicken L-lactate dehydrogenase B chain ELISA Kit
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
96wellplate
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文献和实验Detection of Pyroptosis by Measuring Released Lactate Dehydrogenase Activity
integrity and lyse, releasing lactate dehydrogenase (LDH) that is normally maintained within the cell cytosol. Thus, pyroptosis is distinct from apoptosis, which results in cellular contents being enclosed within membrane blebs during cellular demise. LDH
,and the cell pellets were frozen and stored at –80ºC. Lysis Lysis was accomplished with B-PER reagent supplemented with 1/50 vol 10-mg/ml chicken egg-white lysozyme (stored at –80ºC in aliquots),1/125 vol 5-mg/ml DNaseI (in 20 mM sodium acetate,pH adjusted
T-Cell-Dependent Immune Responses, Germinal Center Development, and the Analysis of V-Gene Sequences
in single GC B cells directly dissected from frozen tissue sections. The V Ox1 L-chain sequences are amplified by polymerase chain reaction (PCR), cloned, and sequenced. Methods are described to analyze V-gene sequences. Potential difficulties
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