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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
Shaking 4 Plate Incubator, 220V, Plug Type G-UK, Ireland
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
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- 规格:
Each
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文献和实验. 4). 10) Place a sterile syringe filter into the air vent (Fig. 4). 11) Move the flow circuit into a humidified incubator at 37 °C, 5% CO2 . 12) Clamp the hard tubing into the roller pump head indicated for use with this type
Construction and Characterization of Adenovirus Vectors
the tubes for 90 sec at 42°C and then for 2 min on ice. 7. To each tube, add 1 mL of LB. Incubate with shaking at 225 rpm for 25 min at 37°C. 8. Transfer the bacteria to 1.5-mL tubes. Centrifuge at 8500g for 1 min at room
medium.f.Incubate for 1 hour in the 37℃ shaking incubator at 200 rpm.g.Using sterile technique, spread 40 µl from each transformation onto a prewarmed LB plate. Let stand right-side up for about 5 minutes to allow the cells to adhere to the agar
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