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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
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- 英文名:
Basic Peristaltic Pump, 10 rollers, 1 channel, Flow range: 0.002-48ml/min, 220V, Plug Type E-Europe
- 库存:
99
- 供应商:
北京孚博生物
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文献和实验. Incubate for 15 min at 37 °C. Rinse each flask x 2 with 10 ml DPBS (without Ca or Mg). 6) Add 4 ml of Trypsin to each flask. Incubate at 37 °C for 3 min. Neutralize with 8 ml of Trypsin Neutralization Solution. 7) Wash
Dynamic Flow Assay in a Parallel Plate Flow Chamber
connected to outlet flow chamber at a shear stress in the range of 0.1-4.0 dynes/cm2 . 7. Allow cells to flow for sufficient time to get an adequate number of cells interacting with the cell monolayer or coated surface. Generally 3-10 min. is used.
Dynamic Flow Assay in a Parallel Plate Flow&n
1 ml of 5.5µg/ml FN solution to each dish. 2. Incubate for 30 min. at room temperature. 3. Aspirate solution out of each dish. 4. Add 2 ml of cell suspension (Huvecs, CHO cells) to each dish at seeding density of 0.5-3.0 x 105 cells/ml
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