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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
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- 英文名：
Liquid Suction Vacuum Pump, 8L/min, 220V, Plug Type I-Australia
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
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文献和实验Protocol for vacuum-infiltration transformation of Arabidopsis thaliana
). Transformation by vacuum infiltration Six mg of WS seeds (about 300 seeds) were sown on compost in 40x30 cm sowing flats . The germination was synchronized by cold treatment for 48 h at 40C, and the flats were placed in greenhouse (16 hours day
and add 2 L of 0.1 M MgSO4. Mix, settle, aspirate, and vacuum filter as in step 1. Transfer the phosphocellulose resin to the 4 L beaker, add 2 L of 10 x column buffer. Mix gently for 10-15 min, allow the resin to settle, aspirate excess column
Mix, settle, aspirate, and vacuum filter as in step 1. Transfer the phosphocellulose resin to the 4 L beaker, add 2 L of 10 x column buffer. Mix gently for 10-15 min, allow the resin to settle, aspirate excess column buffer
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