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- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
Optimizer Buffer™-II
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
2x25ml
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文献和实验Multiplex Integrating Waveguide Sensor: Signalyte™-II
A platform to detect multiplex fluorescent labels was developed based on liquid phase implementation of the Integrating Waveguide Sensor detection principles. The liquid sample is held in a capillary cuvette with a lens at one end
with the better the chances of a successful pulldown. A total of 4.0×106 293 HEK cells (~70% confluent) are plated and 24 hours later transfected with 15µg of a RNA Polymerase II expressing flag-tagged construct plasmid. We generally use Lipofectamine 2000™
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
(most critical timed step). 5. Add 300 ul of cold Qiagen buffer R3 (neutralization buffer) per well. Seal wells with plastic sealing tape and mix by gentle inversion 5 times. Incubate on ice for 5 minutes. 6. Add 50 ul of ProCipitate™ (LigoChem
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