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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 保质期:
12 months
- 级别:
WB相关试剂
- 供应商:
武汉益普生物科技有限公司
- 应用范围:
WB
- 抗体英文名:
Western一抗二抗去除液 (中性)
- 规格:
200mL/500mL
| 规格: | 200mL | 产品价格: | ¥240.0 |
|---|---|---|---|
| 规格: | 500mL | 产品价格: | ¥460.0 |
| 产品名称 | Western一抗二抗去除液 (中性) |
| 产品货号 | E-IR-R102 |
| 应用性 | WB |
| 运输条件 | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| 保存及保质期 | 2~8°C保存,保质期12个月。 |

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文献和实验Probing and Stripping of Western Blot
270 ml H2OStripping the antibody from Western Blot(Natalya Yudkovsky and Jeff Ranish)Note: these two methods both work, but the first is less time consuming:Easy Stripping Method:1. Put the blot in the following solution
1) After ECL development, wash membrane once for 10min with PBST. 2) Incubate the membrane in stripping buffer (see below) in a heat-sealed plastic bag for 30min at 50oC with occasional mixing. For low-abundance antigens, the strip
Stripping for reprobing western blots
minutes. (2) Discard buffer. (3) 5-10 minutes fresh stripping buffer. (4) Discard buffer. (5) 10 minutes PBS (6) 10 minutes PBS (7) 5 minutes TBST (8) 5 minutes TBST Ready
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