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- S0142
- 2025年10月07日
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- 保存条件：
负80摄氏度
- 保质期：
3个月
- 英文名：
Rosetta2(DE3) E.coli Strain
- 库存：
50
- 供应商：
LSM BIO
- 规格：
100ul
Rosetta2(DE3) E.coli Strain
S0142 100ul Store at -80鈩?/p>
Rosetta2(DE3) E.coli Strain Information
Strain classification: Escherichia coli
Strain function: protein expression
Genotype: F-ompT hsdSB(rB-mB-) gal dcm(DE3) pRARE(argU, argW, ilex, glyT, leuW, proL) (CamR)
Resistance: Chl
Culture medium: LB
Culture temperature: 37鈩?/p>
Storage temperature: -80鈩?/p>
Storage method: 20% glycerol bacteria
Rosetta2(DE3) E.coli Strain Genotype
F-ompT hsdSB(rB-mB-) gal dcm(DE3) pRARE(argU, argW, ilex, glyT, leuW, proL) (CamR)
Rosetta2(DE3) E.coli Strain Description
Rosetta2(DE3) E.coli Strain has chloramphenicol resistance and supplements tRNA corresponding to six rare codons (AUA, AGG, AGA, CUA, CCC, GGA) lacking in Escherichia coli, increasing the expression level of exogenous genes, especially eukaryotic genes, in the prokaryotic system. The chromosome of this strain integrates λ The DE3 region of bacteriophage (containing T7 bacteriophage RNA polymerase), which simultaneously expresses T7 RNA polymerase and Escherichia coli RNA polymerase, can be used for protein expression of plasmids such as pET series, pGEX, pMAL, etc
Preparation of 1 M IPTG solution
Add 2.38 g of IPTG to 10 mL of sterile double distilled water, completely dissolve, and filter with a 0.22um filter membrane for sterilization.
Chloramphenicol preparation
Chloramphenicol 100mg/ml is dissolved in ethanol, completely dissolved, and then filtered with a 0.22um filter membrane for sterilization; Working concentration: 34 µ g/ml.
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. This product is glycerol packed, Please kindly culture it as soon as possible then harvest and store at -80鈩?
3. Shipping temperature is 2-8鈩?
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文献和实验Optimizing E. coli-Based Membrane Protein Production Using Lemo21(DE3) and GFP-Fusions
Optimizing the conditions for the overexpression of membrane proteins in E . coli and their subsequent purification is usually a laborious and time-consuming process. Combining the Lemo21(DE3) strain, which conveniently allows to identify
定的原因可能是PrP对E.coli BL21(DE3)具有细胞毒性.pET-32a(+)来源于pBR-322,pBR-322源于ColE1.ColE1、pBR-322 、pET- 32a(+)都失去了分配功能区par.而天然质粒具有功能区par,可以保证质粒在每次细胞周期中准确的进行分离,并均等的分配到子代细胞中去.功能区 par对质粒PrP-pET-32a(+)的稳定性是不可缺少的[4].缺少功能区par的pET-32a(+)质粒在每次细胞周期中随机分配到子代细胞中去,无细胞毒性时约98
. Chromosomal genes carried in the F plasmid are listed in brackets. rB/K +/- = The (B/K) defines the strain lineage. The +/- indicates whether the strain has or hasn't got the restriction system. mB/K +/- = The (B/K
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