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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
Biotin (hydroxyprolinol) CPG 1000, 1 g
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
详询
- 规格:
1g
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文献和实验of choice (15µg, Lipofectamine 2000™ Invitrogen). The following day the flag-tagged protein expressing plasmid transfected cultures are transfected again with 100nM EF52 biotin labeled siRNA (antisense or sense alone) using Lipofectamine 2000™ or stock 3.4µM
expressing plasmid of choice (15µg, Lipofectamine 2000™ Invitrogen). The following day the flag-tagged protein expressing plasmid transfected cultures are transfected again with 100nM EF52 biotin labeled siRNA (antisense or sense alone) using Lipofectamine
Methylated DNA Immunoprecipitation (MeDIP)
against 5-methylcytidine (5mC) [4] (Eurogentec #BI-MECY-1000). A portion of the sonicated DNA should be left untreated to serve as input control. Dilute 4 µg (note 3) of sonicated DNA in 450 µl TE Denature for 10 minutes in boiling water and immediately
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