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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
BDP® TR NHS ester, 50 mg
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
详询
- 规格:
50mg
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文献和实验Labeling Tubulin with Tetramethylrhodamine Succinimidyl Ester
fresh 10 mg/ml stock in DMSO.Need 2.5 mg per 50 mg of tubulin. 8.Potassium glutamate,1 M.Prepare 1 M glutamic acid and titrate with KOH to pH 6.9. 9.Injection Buffer: 50 mM potassium glutamate,0.5 mM MgCl2,pH 6.5-6.7.Prepare 10 ml.Keep ~5 ml at 37℃
LABELING MUSCLE ACTIN WITH CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER
. 4.3 mM CaCl2,1.2 M KI,50 mM Tris-HCl,pH 8.0,5 ml. 5.0.5 mM ATP,0.2 mM CaCl2,0.5 mM DTT,2 mM Tris-HCl,pH 8.0 at 4℃,1 liter. 6.CFSE (Molecular Probes,C-1311; not the diacetate),prepare a stock solution of 50 mg/ml in DMSO. 7.Small SS34 tubes and adaptors
实验步骤 1. Measure out the amount of Taq DNA polymerase needed. When using a reaction cocktail, determine the total number of units in the mix. 2. Add an amount of “equivalent units” of Platinum® Taq
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