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- BPM-BP-40660-25MG
- 2026年02月22日
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文献和实验Labeling Tubulin and Quantifying Labeling Stoichiometry
and stoichiometry for tubulin labeled with tetramethylrhodamine (TMR) NHS ester: Tubulin concentration = [(A280 - Contribution of dye to A280 ) x Dilution Factor] / Extinction coefficient of tubulin at 280 nm TMR concentration = (A555 x Dilution Factor
, NY). Kemptide, I-2, and Elk1 were spotted in quadruplicate on BSA-NHS slides and processed as described above. The slides were then washed 3 times for 10 min each with Wash Buffer (WB; 20 mM Tris, 150 mM NaCl, 10 mM EDTA, 1 mM EGTA, 0.1% Triton X
), provided that immunoreactivity is preserved and that a sufficiently high specific activity is achieved. The fluorescent antigen labeling method presented in this Chapter features the use of N―hydroxysuccinimido ester derivatives of a commercially available
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