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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
C5aR, Mouse, mAb 10/92, 100 ug
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
详询
- 规格:
100UG
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文献和实验mlAdd 40 g ammonium sulfate for every 100 ml Sup. (slowly):800gStir O/N in cold room.Pour into plastic bottles. Spin at 4 ℃, 7,500 rpm for 20 min in JA-10 rotor.Prepare 4L 100 mM Tris/pH7.8 (400 ml of 1 M stock/4 L) or Binding buffer.Discard Sup. Resuspend
g Stir O/N in cold room. Pour into plastic bottles. Spin at 4 o C, 7,500 rpm for 20 min in JA-10 rotor. Prepare 4L 100 mM Tris/pH7.8 (400 ml of 1 M stock/4 L) or Binding buffer . Discard Sup. Resuspend pellets in 10 ml
T-Cell Activation Using mAb to CD3
gives a good response. If experimental conditions require, a titration of cell densities (2-3x106 /ml to 105 /ml) should be performed for optimization. Add 100µl of the cell suspension to each well. For each condition, use triplicate wells.
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