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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
T4 DNA Ligase, 5U/uL *One pouch containing 2 tubes: T4 and 10X buffer
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
500U
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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
(1X PBS containing 0.5 % bovine serum albumin, filtered) T4 DNA ligase (400 U/µL) and 10X buffer Taq polymerase (New England Biolabs) TaqMan PCR master mix (Applied Biosystems) (see Step 38) TE (1X, pH 7.4) Triton X-100
buffer 1 μl T4 DNA ligase (NEB 202L) 1 μl (400 U/ul) sterile ddH2 O q.s. Final volume 10 μl The cloning vector typically is SmaI-linearized, CIAP-dephosphorylated M13RF, and the preparation of this vector is described below. In some instances, SmaI
Preparation of End-Labeled DNA Probes by Conventional Kinase for DNA Footprinting Analysis
Treatment 1. Add 20 μl of 10X CIP Buffer. 2. Add diluted CIP stock to the DNA such that the final concentration is approximately 0.2 Units CIP per μg DNA in a final volume of 200 μl (see Hint #4). 3. Incubate at 37°C
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