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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
Bodi Fluor™ 576/589 NHS Ester (equivalent to BODIPY™ 576/589 NHS Ester)
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
5mg
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文献和实验Labeling Tubulin and Quantifying Labeling Stoichiometry
that are not clear, using DMSO instead of glycerol for the first polymerization step appears to increase the labeling stoichiometry by ~25% for C2CF-SNHS ester. For labeling with rhodamine (tetramethylrhodamine NHS ester) and X-rhodamine, we generally use glycerol
, NY). Kemptide, I-2, and Elk1 were spotted in quadruplicate on BSA-NHS slides and processed as described above. The slides were then washed 3 times for 10 min each with Wash Buffer (WB; 20 mM Tris, 150 mM NaCl, 10 mM EDTA, 1 mM EGTA, 0.1% Triton X
Site‐Specific Protein Labeling with SNAP‐Tags
) Anhydrous N ,N ‐dimethyl formamide (DMF, Sigma‐Aldrich) IRDye 800CW N‐hydroxysuccinimide (NHS) ester (Licor, cat. no. 929–70020; http://www.licor.com
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