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- abx710071-100ml
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store the basal medium at 4 °C, and all other solutions at -20 °C. Avoid exposure to light.
- 保质期：
详询
- 英文名：
【干冰】Medium for Rat Thymic Fibroblasts (TF)
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
100ml
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
of one rat spleen (approx. 10**8 cells) together with 6 x 10**7 Y3/Ag1.2.3 per fusion (the other half of the spleen cells can be frozen as a backup in 90% FCS +10% DMS0). c) Try and keep all cells, PEG and medium components at 37 deg. C
Protocols for the preparation of tumour cells for s.c. injections in mice
are washed with sterile PBS. C51 mouse colon adenocarcinoma cells and FE-8 rat transformed fibroblasts are cultured in flasks with no gelatin coated surface. Media: Cell culturing medium for F9 mouse teratocarcinoma and SKMEL-28 human melanoma
Culture Conditions that Optimize Outgrowth of Hybridomas
of contaminating fibroblasts and macrophages in the primary culture, the concentration of fused and unfused cells present in primary cultures, the presence or absence of essential growth factors in fetal bovine serum, coculturing of fresh hybrids with thymocytes
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