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- Abbexa
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- abx098022-50rxns×20ulSystems
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store at -20°C. Stable for 12 months from date of receipt.
- 保质期：
详询
- 英文名：
【干冰】One-Step gDNA Removal and cDNA Synthesis SuperMix (15kb)
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
50rxns×20ulSystems
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文献和实验cDNA Synthesis from MOLT-4 Cells
second strand yield. 11. Add 0.5 ml PN buffer from Qiegen Nucleotide Removal Kit to the remaining reaction, and mix it ,load it into the spin column, and follow the pr℃edures comimg with the kit to purify the double strand DNA. 12. Elute the cDNA
V for 1-2 h. 3) Stain with EtBr or SYBR® Green II, visualize, and image gel. 4. cDNA Synthesis A protocol for cDNA synthesis using 0.1-2 Kb RNA Ladder is described below. Other protocols are suitable. 1) Incubate 2 μg
Synthesis of Radiolabeled, Subtracted cDNA Probes Using Oligo(dT) as a Primer
dNTPs by chromatography through a 5-ml column of Sephadex G-50. IMPORTANT Perform this step and all subsequent steps with siliconized tubes. 9. To the radiolabeled cDNA, add tenfold excess by weight of the driver RNA that will be used
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