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T25
PK15/PK15细胞系/PK15细胞株/PK15猪肾上皮细胞
Cell line name PK-15
Synonyms PK(15); PK (15); PK 15; PK15; Porcine Kidney-15
Accession CVCL_2160
Resource Identification Initiative To cite this cell line use: PK-15 (RRID:CVCL_2160)
Comments Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).
Virology: PK-15 from ATCC was found to be infected with porcine circovirus type 1 (PCV1) (PubMed=2686830; PubMed=4151202).
Virology: Susceptible to infection by SARS coronavirus (SARS-CoV) (PubMed=15731278).
Doubling time: 12.1 +- 0.7 hours (PubMed=17889922); ~25 hours (DSMZ=ACC-640).
Omics: Transcriptome analysis by microarray.
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Species of origin Sus scrofa (Pig) (NCBI Taxonomy: 9823)
Breed/subspecies: Hampshire; VBO=VBO_0001146.
Hierarchy Parent: CVCL_S287 (PK-2a)
Children:
CVCL_B0NA (PK-15/A11) CVCL_A5AA (PK-15(CD163)) CVCL_JF19 (PK-15A)
CVCL_YB14 (PK-15S) CVCL_YE75 (PK15 PERV-inactivated clone 15) CVCL_YE76 (PK15 PERV-inactivated clone 20)
Sex of cell Male
Age at sampling Adult
Category Spontaneously immortalized cell line
Publications
PubMed=5962858
Pirtle E.C.
Variation in the modal chromosome number of two PK-15 porcine kidney cell lines.
Am. J. Vet. Res. 27:747-749(1966)
DOI=10.1007/BF02618370
Stulberg C.S., Coriell L.L., Kniazeff A.J., Shannon J.E.
The animal cell culture collection.
In Vitro 5:1-16(1970)
PubMed=4132403; DOI=10.1016/0042-6822(74)90141-X
Todaro G.J., Benveniste R.E., Lieber M.M., Sherr C.J.
Characterization of a type C virus released from the porcine cell line PK(15).
Virology 58:65-74(1974)
PubMed=4151202
Tischer I., Rasch R., Tochtermann G.
Characterization of papovavirus-and picornavirus-like particles in permanent pig kidney cell lines.
Zentralbl. Bakteriol. Orig. A. 226:153-167(1974)
PubMed=6160900; PMCID=PMC1320103
Fulton R.W., Pearson N.J.
Interferon production by bovine tracheal organ cultures infected with bovid herpesvirus 1 strains.
Can. J. Comp. Med. 44:447-452(1980)
PubMed=6988327; DOI=10.1007/BF02831503
O'Brien S.J., Shannon J.E., Gail M.H.
A molecular approach to the identification and individualization of human and animal cells in culture: isozyme and allozyme genetic signatures.
In Vitro 16:119-135(1980)
PubMed=3009166; DOI=10.1507/endocrj1954.32.819
Kinoshita Y., Fukase M., Takenaka M., Nakada M., Miyauchi A., Fujita T.
Calcitonin-responsive clonal cell line from porcine kidney (PK(15)) in serum-free medium.
Endocrinol. Jpn. 32:819-828(1985)
PubMed=3173126
Buonavoglia C., Falcone E., Pestalozza S., Iovane G., Rivero V.B.
Susceptibility of a minipig kidney cell line (MPK) to hog cholera virus.
Microbiologica 11:263-264(1988)
PubMed=2686830; PMCID=PMC1255571
Dulac G.C., Afshar A.
Porcine circovirus antigens in PK-15 cell line (ATCC CCL-33) and evidence of antibodies to circovirus in Canadian pigs.
Can. J. Vet. Res. 53:431-433(1989)
PubMed=15731278; DOI=10.1128/JVI.79.6.3846-3850.2005; PMCID=PMC1075706
Mossel E.C., Huang C., Narayanan K., Makino S., Tesh R.B., Peters C.J.
Exogenous ACE2 expression allows refractory cell lines to suppon.
J. Virol. 79:3846-3850(2005)
PubMed=17889922; DOI=10.1016/j.virol.2007.08.014
Zhu Y., Lau A.H.-L., Lau J.S.-K., Jia Q., Karuppannan A.K., Kwang J.H.-S.
Enhanced replication of porcine circovirus type 2 (PCV2) in a homogeneous subpopulation of PK15 cell line.
Virology 369:423-430(2007)
PubMed=19508353; DOI=10.1111/j.1744-313X.2009.00853.x
Ho C.S., Franzo-Romain M.H., Lee Y.J., Lee J.H., Smith D.M.
Sequence-based characterization of swine leucocyte antigen alleles in commercially available porcine cell lines.
Int. J. Immunogenet. 36:231-234(2009)
PubMed=19937495; DOI=10.1080/10495390903139950
Uebing-Czipura A.U., Dawson H.D., Rutherford M.S., Scherba G.
Transcriptome profile and cytogenetic analysis of immortalized neuronally restricted progenitor cells derived from the porcine olfactory bulb.
Anim. Biotechnol. 20:186-215(2009)
PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Karger A., Bettin B., Lenk M., Mettenleiter T.C.
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)
PubMed=24398227; DOI=10.1016/j.vetmic.2013.12.001; PMCID=PMC3969584
Lithgow P.E., Takamatsu H., Werling D., Dixon L.K., Chapman D.
Correlation of cell surface marker expression with African swine fever virus infection.
Vet. Microbiol. 168:413-419(2014)
PubMed=26456528; DOI=10.1126/science.aad1191
Yang L.-H., Guell M., Niu D., George H., Lesha E., Grishin D., Aach J., Shrock E., Xu W.-H., Poci J., Cortazio R., Wilkinson R.A., Fishman J.A., Church G.M.
Genome-wide inactivation of porcine endogenous retroviruses (PERVs).
Science 350:1101-1104(2015)
PubMed=31216434; DOI=10.1016/j.jviromet.2019.113682
Li Y.-Y., Wang Y.-J., Zhou X.-H., Zhang X.-K., Zhang X.-Y., Xia X.-L., Sun H.-C.
Generation of PK-15 cell lines highly permissive to porcine circovirus 2 infection by transposon-mediated interferon-gamma gene transfer.
J. Virol. Methods 271:113682.1-113682.6(2019)
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文献和实验*发表【中文论文】请标注：由博辉生物科技（广州）有限公司提供； *发表【英文论文】请标注：From Bohui Biological Technology (Guangzhou) Co., Ltd.
我和师兄是从04年初就开始用LLC-PK1细胞系筛选多种类型的、数量极多的各种可能有用的新药，摸索发现消化液的配方为0.25％的胰酶+0.02%EDTA 就行了，要配在PBS中，如果能加Hepes那就更保险了（有一次实验室Hepes用光了，配液时没加Hepes，照样没问题） 消化步骤：吸走培液后最好用PBS过一遍－>50ML培养瓶加2ML在37ºC预热消化液－>37ºC温箱放置1Min－>显微镜下观察，细胞松散开，但尚未脱落时，及时吸走消化液－>必要时可在吸走消化液的情况下，继续放置一会
DNA EXTRACTION FROM MICRODISSECTED PARAFFIN SECTIONS
. Day 1: DISSECTION: See photos of H&E , methyl green before dissection , and methyl green after dissection . Label the top of 0.5 ml pcr tubes, color code dot label them also. Add 15 ul of 1X PCR/PK Buffer into each tube, close caps
Steady State ATPase Assays Coupled Enzyme System
Motor protein (>95% purity; 15-20 µM) Cuvettes (200 µL volume, 10 mm path length) 0.5 M Tris-OAc, pH 7.5 10 mM MgCl2 10 mM DTT
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